- SynonymPPIB,CYPB,PPIase B,CYP-S1
- SourceHuman PPIB, His Tag (PPB-H5229) is expressed from human 293 cells (HEK293). It contains AA Asp 34 - Ala 212 (Accession # NP_000933).Predicted N-terminus: Asp 34Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.7 kDa. The protein migrates as 22-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human PPIB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundPeptidyl-prolyl cis-trans isomerase B (PPIB or PPIase B) is also known as Cyclophilin B (CYPB), Rotamase B, S-cyclophilin (SCYLP), is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum, which belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIB / CYPB contains one PPIase cyclophilin-type domain. PPIases accelerate the folding of proteins and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
- References
- (1)Arber S., et al, 1992, J. Cell Biol. 116:113-125.
- (2)Chi A., et al., 2006, J. Proteome Res. 5:3135-3144.
- (3)Zhang, Jianying, et al., 2003, J. Biol. Chem. (United States) 278 (9): 7459–68.
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