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顶生系统/生物素化人CTGF/CCN2蛋白，His，Avitag™ (推荐用于生物淘洗）/200ug（100ug×2）/CTF
- SynonymCCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8
- SourceBiotinylated Human CTGF, His,Avitag (recommended for biopanning) (CTF-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1).Predicted N-terminus: Gln 27Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 39.2 kDa. The protein migrates as 40-42 kDa and 43-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Potassium glutamate and Arginine, pH7.0. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human CTGF, His,Avitag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human CTGF, His,Avitag (recommended for biopanning) (Cat. No. CTF-H82E6) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.6-5 ng/mL (QC tested).
- BackgroundConnective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
