>
产品中心 >
Other_animal_antigens >
Acrobosystems/人CEACAM-5/CD66e蛋白，His标签（MALS验证）/1mg（500ug×2）/CE5
- SynonymCEACAM-5,CD66e,CEA,Meconium antigen 100
- SourceHuman CEACAM-5, His Tag (CE5-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731).Predicted N-terminus: Lys 35Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 72.2 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CEACAM-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CEACAM-5, His Tag (Cat. No. CE5-H5226) was more than 95% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.
Immobilized Human CEACAM-5, His Tag (Cat. No. CE5-H5226) at 1 μg/mL (100 μL/well) can bind Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Immobilized Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 Antibody at 1 μg/mL (100 μL/well) can bind Human CEACAM-5, His Tag (Cat. No. CE5-H5226) with a linear range of 10-39 ng/mL (Routinely tested).
- BackgroundCarcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
