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ACROBiosystems/Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)/1mg/CDD
- SynonymCD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
- SourceHuman CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-H52W0) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)).Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)Request for sequence
- Molecular Characterization
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E) and 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with flag-tagged human IgG1 Fc fragment at the C-terminus. The reducing (R) heterodimer protein migrates as 40-55 kDa due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) was more than 90% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.
Immobilized Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) at 2 μg/mL (100 μL/well) can bind OKT3 (mouse IgG2a) (Cat. No. CDE-M120a) with a linear range of 10-78 ng/mL (QC tested).
Immobilized Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind OKT3 (mouse IgG2a) with an affinity constant of 31.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
- BackgroundT-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
