- SynonymCTSS,Cathepsin S
- SourceHuman Cathepsin S, His Tag (CTS-H5224) is expressed from human 293 cells (HEK293). It contains AA Gln 17 - Ile 331 (Accession # P25774-1).Predicted N-terminus: Gln 17 (pro) & Leu 115 (mature)Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the propeptide and mature chain. The propeptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 36.8 kDa and 24.9 kDa respectively. The protein migrates as 36-42 kDa (propeptide) and 26 kDa (mature) under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM MES, 100 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Cathepsin S, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundCathepsin S is also known as CTSS, which is a member of the peptidase C1 family. Cathepsin S is a lysosomal cysteine protease that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. Cathepsin S can function as an elastase over a broad pH range in alveolar macrophages. The catalytic activity of cathepsin S is similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.
- References
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