- SynonymTNFRSF17,CD269,BCM,BCMA
- SourceHuman BCMA, Fc Tag (HPLC verified) (BC7-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 1 -Ala 54 (Accession # NP_001183.2).Predicted N-terminus: Met 1Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 32.0 kDa. The protein migrates as 34-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 0.1 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Human BCMA, Fc Tag (HPLC verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human BCMA, Fc Tag (Cat. No. BC7-H5254) was greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human BAFF, His,Avitag (Cat. No. BAF-H82Q2) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with a linear range of 0.1-0.8 ng/mL (QC tested).
Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.1-1.6 ng/mL (Routinely tested).
Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with a linear range of 0.1-2 ng/mL (Routinely tested).
Loaded Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) on HIS1K Biosensor, can bind Human BCMA, Fc Tag (Cat. No. BC7-H5254) with an affinity constant of 1.88 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS Analysis of Anti-BCMA CAR Expression
293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with B. human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BC7-H5254, 3 μg/ml) and C. human Fc tag protein control, followed by FITC-conjugated anti-human IgG Fc antibody. A. Non-transfected 293 cells and C. human Fc tag protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BC7-H5254).
- Citations
Hypoxia Selectively Impairs CAR-T Cells In Vitro
Authors: Berahovich R, et al
Journal: Cancers (Basel) 2019
Application: Flow Cytometry
Request for Full-text
CAR-T cells based on novel BCMA monoclonal antibody block multiple myeloma cell growth.
Authors: Berahovich R, et al.
Journal: Cancers (Basel) 2018
Application: Flow Cytometry
Request for Full-text
- BackgroundTumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
- References
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