- SynonymTNFRSF17,CD269,BCM,BCMA
- SourceFITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) is expressed from human HEK293 cells. It contains AA Met 1 - Ala 54 (Accession # Q02223-1). It is the FITC labeled form of Human BCMA, Fc Tag (Cat. No. BC7-H5254).Predicted N-terminus: Met 1Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 32.0 kDa. As a result of glycosylation, the protein migrates as 35-44 kDa under reducing (R) condition, and 60-75 kDa under non-reducing (NR) condition (SDS-PAGE).
- Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
- LabelingThe primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
- FITC:Protein RatioThe FITC to protein molar ratio is 1.5-3.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>92% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
FITC-Labeled Human BCMA Protein, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 10 μg/mL (100 μL/well) can bindFITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) with a linear range of 0.04-1.25 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human BCMA Protein, Fc Tag (Cat. No. BCA-HF254) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
- BackgroundTumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
- References
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