- SynonymApolipoprotein A-II,APOA2,Apo-AII,ApoA-II,Apolipoprotein A2
- SourceHuman Apolipoprotein A-II, His Tag (APA-H51H6) is expressed from E.coli cells. It contains AA Ala 19 - Gln 100 (Accession # P02652-1).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 11.3 kDa. The protein migrates as 11 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Apolipoprotein A-II, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundApolipoprotein A-II(APO-A2) stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Recent studies have established the interaction between APOA2 -256T>C polymorphism and dietary saturated fatty acids intake in relation to obesity on healthy individuals. Significant interaction between APOA2-saturated fatty acids intake and body mass index (BMI). The result of this study confirmed the interaction between APOA2 -256T>C polymorphism and SFAs intake with BMI in type 2 diabetic patients.
- References
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